Shyam V. Vaidya
Abbott Laboratories, USA
Title: Protein antifouling properties of a chemical vapor deposited alkyl-functional carboxysilane coating characterized using quartz crystal microbalance
Biography
Biography: Shyam V. Vaidya
Abstract
The protein resistant properties of a chemical vapor deposited alkyl-functional carboxysilane coating (Dursan®) were compared to that of an amorphous fluoropolymer (AF1600) coating and stainless steel by studying non-specific adsorption of various proteins onto the coating surfaces using quartz crystal microbalance with dissipation monitoring (QCM-D). A wash solution with non-ionic surfactant, polyoxyethyleneglycol dodecyl ether (or Brij 35), facilitated 100% removal of residual bovine serum albumin (BSA), mouse immunoglobulin G (IgG), and normal human plasma proteins from the Dursan surface, whereas these proteins remained adsorbed on the bare stainless steel surface. Mechanical stress in the form of sonication demonstrated robustness of the Dursan coating to mechanical wear and showed no impact on the coating’s ability to prevent adsorption of plasma proteins. Surface delamination was observed in case of the sonicated AF1600 coatings and it led to adsorption of plasma proteins. The combination of the robust alkyl-functional carboxysilane coating (Dursan) and non-ionic surfactant in the wash buffer that we have reported here is certainly a step forward toward mitigation of surface biofouling in biotechnological applications, specifically in case of automated immunoassay analyzers, reagent manufacturing, and filling setups.