Nana Gorgaslidze
Tbilisi State Medical University, Georgia
Title: Development of method for quantitative determination of bromelain in gel formulation
Biography
Biography: Nana Gorgaslidze
Abstract
Gel of bromelain has anti-inflammatory properties and hence is used for treatment of edema and inflammations. This was developed at TSMU Iovel Kutateladze Institute of Pharmacochemistry. On previous researches we published the data of some properties of papain and bromelain containing gels. The presented work belongs to the development and validation of accurate and sensitive assay method for quantitative determination of bromelain in gel based on UV-spectrophotometric analysis. Elastasin was used as substrates for their analysis. Absorbance was recorded at 595 nm. The developed method is linear, precise and sensitive; Intra- and inter-day measurements. All methods were validated as per ICH guidelines and can be adopted for the routine analysis of bromelain in gel formulations.